The gambling game has long been entwined in Australian military history and is traditionally played at RSL Clubs across Queensland on Anzac Day, despite it previously being technically illegal.
The Charitable and Non-Profit Gaming (Two-Up) Amendment Bill 2011 passed Parliament in February with bipartisan support.
Former attorney-general Paul Lucas said the new law formally allowed punters to play not-for-profit two-up in RSL and Services clubs.
The changes were welcome news for ex-servicemen Brian Evans, Tom Burton and John Paul of the Goodna RSL, who would no longer be flouting the law this Anzac Day.
Goodna RSL president David Christie said veterans across the state would welcome the changes.
"We're looking forward to having the veterans come into the club on Anzac Day to enjoy a drink and meal with the members of the club and also to enjoy a game of two-up and crown and anchor," he said.
"It takes away the police's authority to actually charge someone for playing the game that has been a tradition of the Diggers."